2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Mga estandard nga substansiya nga gigamit sa kurba sa kalibrasyon sa relatibong distribusyon sa masa sa molekula: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrumento ug kagamitan
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Sa kinatibuk-an, ang proporsiyon sa mga amino acid sa mga produkto sa Sustar mas taas kay sa mga produkto sa Zinpro.
Bahin 8 Mga Epekto sa Paggamit
Mga epekto sa lain-laing tinubdan sa trace minerals sa performance sa produksiyon ug kalidad sa itlog sa mga himungaan sa ulahing panahon sa pagpangitlog
Proseso sa Produksyon
Gipunting nga teknolohiya sa chelation
Teknolohiya sa shear emulsification
Teknolohiya sa pag-spray ug pagpauga sa presyur
Teknolohiya sa pagpabugnaw ug pag-dehumidipikasyon
Abansado nga teknolohiya sa pagkontrol sa kalikopan
Apendiks A: Mga Pamaagi para sa Pagtino sa relatibong molekular nga distribusyon sa masa sa mga peptide
Pagsagop sa sumbanan: GB/T 22492-2008
1 Prinsipyo sa Pagsulay:
Gitino kini pinaagi sa high performance gel filtration chromatography. Buot ipasabot, gamit ang porous filler isip stationary phase, base sa kalainan sa relative molecular mass size sa mga sample components para sa separation, nga nakita sa peptide bond sa ultraviolet absorption wavelength nga 220nm, gamit ang dedicated data processing software para sa pagtino sa relative molecular mass distribution pinaagi sa gel filtration chromatography (ie, ang GPC software), ang mga chromatogram ug ang ilang data giproseso, gikalkulo aron makuha ang gidak-on sa relative molecular mass sa soybean peptide ug ang distribution range.
2. Mga Reagent
Ang eksperimental nga tubig kinahanglan nga makatuman sa espesipikasyon sa sekondaryang tubig sa GB/T6682, ang paggamit sa mga reagent, gawas sa mga espesyal nga probisyon, kinahanglan nga analitikal nga putli.
2.1 Ang mga reagent naglakip sa acetonitrile (puro sa chromatographic), trifluoroacetic acid (puro sa chromatographic),
2.2 Mga estandard nga substansiya nga gigamit sa kurba sa kalibrasyon sa relatibong distribusyon sa masa sa molekula: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrumento ug kagamitan
3.1 High Performance Liquid Chromatograph (HPLC): usa ka chromatographic workstation o integrator nga adunay UV detector ug GPC data processing software.
3.2 Yunit sa pagsala ug pag-degassing sa vacuum sa mobile phase.
3.3 Elektronikong balanse: gradwadong kantidad nga 0.000 1g.
4 Mga lakang sa operasyon
4.1 Mga kondisyon sa kromatograpiko ug mga eksperimento sa pagpahiangay sa sistema (mga kondisyon sa reperensya)
- 4.1.1 Kolum nga kromatograpiko: TSKgelG2000swxl300 mm×7.8 mm (sulud nga diyametro) o uban pang mga kolum nga gel nga parehas og klase nga adunay parehas nga performance nga angay alang sa pagtino sa mga protina ug peptide.
- 4.1.2 Hugna sa paglihok: Acetonitrile + tubig + trifluoroacetic acid = 20 + 80 + 0.1.
- 4.1.3 Haba sa balud sa pag-ila: 220 nm.
- 4.1.4 Gikusgon sa pag-agos: 0.5 mL/min.
- 4.1.5 Oras sa pag-ila: 30 min.
- 4.1.6 Gidaghanon sa sampol nga ineksiyon: 20μL.
- 4.1.7 Temperatura sa kolum: temperatura sa kwarto.
- 4.1.8 Aron ang sistema sa chromatographic makatuman sa mga kinahanglanon sa pag-ila, gikasabutan nga ubos sa nahisgutang mga kondisyon sa chromatographic, ang kahusayan sa gel chromatographic column, i.e., ang teoretikal nga gidaghanon sa mga plato (N), dili moubos sa 10000 nga gikalkulo base sa mga taluktok sa tripeptide standard (Glycine-Glycine-Glycine).
- 4.2 Paghimo sa mga standard curve sa relatibong molekular nga masa
- Ang nahisgutang lain-laing relative molecular mass peptide standard solutions nga adunay mass concentration nga 1 mg/mL giandam pinaagi sa mobile phase matching, gisagol sa usa ka piho nga proporsyon, ug dayon gisala pinaagi sa organic phase membrane nga adunay pore size nga 0.2 μm~0.5 μm ug gi-inject sa sample, ug dayon nakuha ang mga chromatogram sa mga standard. Ang relative molecular mass calibration curves ug ang ilang mga equation nakuha pinaagi sa pag-plot sa logarithm sa relative molecular mass batok sa retention time o pinaagi sa linear regression.
4.3 Pagtambal gamit ang sampol
Tukma nga timbangon ang 10mg nga sample sa usa ka 10mL nga volumetric flask, butangi og gamay nga mobile phase, ultrasonic shaking sulod sa 10min, aron ang sample hingpit nga matunaw ug masagol, lasawon sa mobile phase hangtod sa timbangan, ug dayon isala pinaagi sa organic phase membrane nga adunay pore size nga 0.2μm~0.5μm, ug ang filtrate gi-analisar sumala sa mga kondisyon sa chromatographic sa A.4.1.
- 5. Kalkulasyon sa relatibong molekular nga distribusyon sa masa
- Human sa pag-analisar sa solusyon sa sample nga giandam sa 4.3 ubos sa mga kondisyon sa chromatographic sa 4.1, ang relatibong molekular nga masa sa sample ug ang distribusyon niini makuha pinaagi sa pag-ilis sa datos sa chromatographic sa sample ngadto sa calibration curve 4.2 gamit ang GPC data processing software. Ang distribusyon sa relatibong molekular nga masa sa lain-laing mga peptide mahimong makalkulo pinaagi sa peak area normalization method, sumala sa pormula: X=A/A total×100
- Sa pormula: X - Ang tipik sa masa sa usa ka relatibong molekular nga masa nga peptide sa kinatibuk-ang peptide sa sample, %;
- A - Kinatas-ang gilapdon sa usa ka relatibong molekular nga masa nga peptide;
- Kinatibuk-ang A - ang sumada sa mga peak area sa matag relatibong molecular mass peptide, nga gikalkulo hangtod sa usa ka decimal place.
- 6 Pagkabalik-balik
- Ang hingpit nga kalainan tali sa duha ka independente nga mga determinasyon nga nakuha ubos sa mga kondisyon sa pagkabalik-balik dili molapas sa 15% sa aritmetikong mean sa duha ka determinasyon.
- Apendiks B: Mga Pamaagi sa Pagtino sa Libre nga mga Amino Acid
- Pagsagop sa sumbanan: Q/320205 KAVN05-2016
- 1.2 Mga reagent ug materyales
- Glacial acetic acid: analitikal nga puro
- Asido nga perchloric: 0.0500 mol/L
- Indikasyon: 0.1% kristal nga violet nga indikasyon (glacial acetic acid)
- 2. Pagtino sa libre nga mga amino acid
Ang mga sample gipauga sa 80°C sulod sa 1 ka oras.
Ibutang ang sampol sa usa ka uga nga sudlanan aron natural nga mobugnaw sa temperatura sa kwarto o pabugnawa sa magamit nga temperatura.Timbanga ang gibana-bana nga 0.1 g nga sample (tukma sa 0.001 g) ngadto sa 250 mL nga uga nga conical flask.Dali nga ipadayon ang sunod nga lakang aron malikayan ang pagsuhop sa sample sa kaumog sa palibotIdugang ang 25 mL nga glacial acetic acid ug isagol og maayo sulod sa dili molapas sa 5 ka minuto.Idugang ang 2 ka tulo sa crystal violet indicatorI-titrate gamit ang 0.0500 mol / L (±0.001) nga standard titration solution sa perchloric acid hangtod nga ang solusyon mausab gikan sa purpura ngadto sa katapusan nga punto.
Irekord ang gidaghanon sa standard nga solusyon nga nahurot.
- Buhata ang blank test sa samang higayon.
- 3. Kalkulasyon ug mga resulta
- Ang sulod sa libreng amino acid nga X sa reagent gipahayag isip mass fraction (%) ug gikalkulo sumala sa pormula: X = C × (V1-V0) × 0.1445/M × 100%, sa pormula:
- C - Konsentrasyon sa standard nga solusyon sa perchloric acid sa moles kada litro (mol/L)
- V1 - Volume nga gigamit para sa titration sa mga sample gamit ang standard perchloric acid solution, sa milliliters (mL).
- Vo - Volume nga gigamit para sa titration blank gamit ang standard perchloric acid solution, sa milliliters (mL);
M - Misa sa sampol, sa gramo (g).
| 0.1445: Kasagarang masa sa mga amino acid nga katumbas sa 1.00 mL sa standard nga perchloric acid solution [c (HClO4) = 1.000 mol / L]. | 4.2.3 Cerium sulfate standard titration solution: konsentrasyon c [Ce (SO4) 2] = 0.1 mol/L, giandam sumala sa GB/T601. | |
| Pagsagop sa mga sumbanan: Q/70920556 71-2024 | 1. Prinsipyo sa pagtino (Fe isip pananglitan) | Ang mga amino acid iron complex adunay ubos kaayo nga solubility sa anhydrous ethanol ug ang mga free metal ions matunaw sa anhydrous ethanol, ang kalainan sa solubility tali sa duha sa anhydrous ethanol gigamit aron mahibal-an ang chelation rate sa mga amino acid iron complex. |
| Sa pormula: V1 - gidaghanon sa cerium sulfate standard solution nga gigamit para sa titration sa test solution, mL; | Anhydrous ethanol; ang nahabilin parehas sa clause 4.5.2 sa GB/T 27983-2011. | 3. Mga Lakang sa Pag-analisar |
| Paghimo og duha ka pagsulay nga dungan. Timbanga ang 0.1g sa sample nga gipauga sa 103±2℃ sulod sa 1 ka oras, nga may katukma nga 0.0001g, idugang ang 100mL nga anhydrous ethanol aron matunaw, isala, isala ang nahabilin nga gihugasan gamit ang 100mL nga anhydrous ethanol labing menos tulo ka beses, dayon ibalhin ang nahabilin ngadto sa 250mL nga conical flask, idugang ang 10mL nga sulfuric acid solution sumala sa clause 4.5.3 sa GB/T27983-2011, ug dayon buhata ang mosunod nga mga lakang sumala sa clause 4.5.3 nga “Initon aron matunaw ug dayon pabugnawa” sa GB/T27983-2011. Buhata ang blank test sa samang higayon. | 4. Pagtino sa kinatibuk-ang sulod sa puthaw | 4.1 Ang prinsipyo sa pagtino parehas sa clause 4.4.1 sa GB/T 21996-2008. |
4.2. Mga Reagent ug Solusyon
| 4.2.1 Sinagol nga asido: Idugang ang 150mL nga sulfuric acid ug 150mL nga phosphoric acid sa 700mL nga tubig ug isagol og maayo. | 4.2.2 Solusyon sa timailhan sa sodium diphenylamine sulfonate: 5g/L, giandam sumala sa GB/T603. | 4.2.3 Cerium sulfate standard titration solution: konsentrasyon c [Ce (SO4) 2] = 0.1 mol/L, giandam sumala sa GB/T601. | |
| 4.3 Mga Lakang sa Pag-analisar | Paghimo og duha ka pagsulay nga dungan. Timbanga ang 0.1g nga sample, tukma sa 020001g, ibutang sa 250mL nga conical flask, idugang ang 10mL nga mixed acid, human matunaw, idugang ang 30ml nga tubig ug 4 ka tulo sa sodium dianiline sulfonate indicator solution, ug dayon buhata ang mosunod nga mga lakang sumala sa clause 4.4.2 sa GB/T21996-2008. Buhata ang blank test sa samang higayon. | 4.4 Representasyon sa mga resulta | Ang kinatibuk-ang iron content X1 sa amino acid iron complexes sa termino sa mass fraction sa iron, ang bili nga gipahayag sa %, gikalkulo sumala sa pormula (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - cerium sulfate standard solution nga gigamit para sa titration sa blankong solusyon, mL; | V0 - cerium sulfate standard solution nga gigamit para sa titration sa blankong solusyon, mL; | C - Aktwal nga konsentrasyon sa cerium sulfate standard solution, mol/L5. Pagkalkulo sa iron content sa chelatesAng iron content nga X2 sa chelate kon itandi sa mass fraction sa iron, ang bili nga gipahayag sa %, gikalkulo sumala sa pormula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Sa pormula: V1 - gidaghanon sa cerium sulfate standard solution nga gigamit para sa titration sa test solution, mL; | V2 - cerium sulfate standard solution nga gigamit para sa titration sa blanko nga solusyon, mL;nom1-Misa sa sampol, g. Kuhaa ang aritmetikong mean sa mga resulta sa parallel determination isip mga resulta sa determinasyon, ug ang hingpit nga kalainan sa mga resulta sa parallel determination dili molapas sa 0.3%. | 0.05585 - masa sa ferrous iron nga gipahayag sa gramo nga katumbas sa 1.00 mL sa cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Misa sa sampol, g. Kuhaa ang aritmetikong mean sa mga resulta sa parallel determination isip mga resulta sa determinasyon, ug ang hingpit nga kalainan sa mga resulta sa parallel determination dili molapas sa 0.3%. | 6. Pagkalkulo sa gikusgon sa chelationRate sa Chelation X3, ang bili nga gipahayag sa %, X3 = X2/X1 × 100Apendiks C: Mga Pamaagi sa Pagtino sa Chelation Rate sa Zinpro |
Pagsagop sa sumbanan: Q/320205 KAVNO7-2016
1. Mga reagent ug materyales
a) Glacial acetic acid: analitikal nga puro; b) Perchloric acid: 0.0500mol/L; c) Indikasyon: 0.1% crystal violet indicator (glacial acetic acid)
2. Pagtino sa libre nga mga amino acid
2.1 Ang mga sampol gipauga sa 80°C sulod sa 1 ka oras.
2.2 Ibutang ang sampol sa usa ka uga nga sudlanan aron natural nga mobugnaw sa temperatura sa kwarto o pabugnawa sa magamit nga temperatura.
2.3 Timbanga ang gibana-bana nga 0.1 g nga sample (tukma hangtod sa 0.001 g) ngadto sa 250 mL nga uga nga conical flask
2.4 Dali nga ipadayon ang sunod nga lakang aron malikayan nga masuhop sa sampol ang kaumog sa palibot.
2.5 Idugang ang 25mL nga glacial acetic acid ug isagol og maayo sulod sa dili molapas sa 5 ka minuto.
2.6 Idugang ang 2 ka tulo sa crystal violet indicator.
2.7 Titrate gamit ang 0.0500mol/L (±0.001) nga standard titration solution sa perchloric acid hangtud nga ang solusyon mausab gikan sa purpura ngadto sa berde sulod sa 15 segundos nga dili mausab ang kolor isip katapusan nga punto.
2.8 Irekord ang gidaghanon sa standard nga solusyon nga nahurot.
2.9 Buhata ang blangko nga pagsulay sa samang higayon.
- 3. Kalkulasyon ug mga resulta
- Katalan
- Physicochemical parameters
V1 - Volume nga gigamit para sa titration sa mga sample gamit ang standard perchloric acid solution, sa milliliters (mL).
Vo - Volume nga gigamit para sa titration blank gamit ang standard perchloric acid solution, sa milliliters (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
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Mga Produkto
Mga mineral nga dili organiko
- Organikong mga mineral nga trace
- Swahili
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Profile sa Kompanya
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | I-klik para sa pangutana | © Copyright - 2010-2025 : Tanang Katungod Gireserba. | Mapa sa Site PANGITA NGA TOP Telepono |
| Tel. | 86-18880477902 | Javanese | E-mail |
| 8618880477902 | Intsik | Pranses | |
| Bird | Intsik | Pranses | Aleman Kinatsila |
| Aquatic animals | Hapon | Koreano | Arabiko Griyego |
| Turko | Italyano | ||
| Ruminant animal g/head day | January 0.75 | Indones Afrikaans Sweko |
Polish
- Basque
- Katalan
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgarian
- Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kroato
Olandes
| Application object | Urdu Vietnamese | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Haitian | Hausa | Kinyarwanda Hmong Hungariano |
| Piglets and fattening pigs | Igbo | Javanese | Kannada Khmer Kurd |
| Kirghiz | Latin | ||
| Bird | 300～400 | 45～60 | Macedonian Malayo Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norwegian
- Pashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbiano
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahili
Tajik
Tamil
Telugu
Thai
| Application object | Urdu Vietnamese | Content in full-value feed (mg/kg) | Efficacy |
| Yiddish | Yoruba | Zulu | Kinyarwanda Oriya Mga Turkmen |
| Uyghur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
